Induced pluripotent stem cells (IPSCs) hold great promise in regenerative medicine, personalized medicine as well as drug discovery. However, while avoiding the ethical controversies associated with embryonic stem cells, they carry neoplastic risk owing to the use of the oncogenes c-Myc as well as Lin28. that will has limited their utility from the biomedical arena.
Work has previously demonstrated that will IPSC generation can be uncoupled coming from c-Myc, yet until currently a viable oncogene- as well as virus-free method has proven elusive.
A fresh research article coming from Alan B Moy as well as colleagues at Cellular Engineering Technologies (IA, USA) as well as The John Paul II Medical Research Institute (IA, USA) describes a promising fresh IPSC reprogramming approach that will attempts to solve these issues. The open access article, “Efficient method to create integration-free, virus-free, Myc as well as Lin28-free human induced pluripotent stem cells coming from adherent cells”, can be available in Future Science OA.
Their approach saw adherent fibroblasts reprogrammed using a combination of reprogramming molecules as well as episomal vectors. The combinatorial approach was successful, yielding colonies in which 100% expressed SSEA4.
“Our reprogramming method provides patient- as well as disease-specific IPSC[s] for drug discovery as well as personalized medicine applications with lower risk of oncogenic perturbations due to Lin28 as well as Myc”, noted the authors. “The reprogramming method paves a pathway for autologous as well as allogeneic cell therapy that will satisfies regulatory requirements.”
The team anticipates that will virus- as well as oncogene-free IPSCs could advance cell therapies, diagnostics as well as personalized medicine. Furthermore, they envision the technology helping to reduce clinical trial failure rates as well as improve drug development.
Stem cell reprogramming factor controls change in cellular energy generation
Anant Kamath et al, Efficient method to create integration-free, virus-free,as well as-free human induced pluripotent stem cells coming from adherent cells, Future Science OA (2017). DOI: 10.4155/fsoa-2017-0028