Beyond just promise, CRISPR can be delivering within the lab today

Precision editing DNA allows for some amazing applications. Credit: Ian Haydon, CC BY-ND

There’s a revolution happening in biology, as well as its name can be CRISPR.


CRISPR (pronounced “crisper”) can be a powerful technique for editing DNA. the item has received an enormous amount of attention within the scientific as well as favorite press, largely based on the promise of what This particular powerful gene editing technology will someday do.

CRISPR was Science magazine’s 2015 Breakthrough of the Year; the item’s been featured prominently within the completely new Yorker more than once; as well as The Hollywood Reporter revealed which Jennifer Lopez will be the executive producer on an upcoming CRISPR-themed NBC bio-crime drama. Not bad for a molecular biology laboratory technique.

CRISPR can be not the first molecular tool designed to edit DNA, although the item gained its fame because the item solves some longstanding problems within the field. First, the item can be highly specific. When properly set up, the molecular scissors which make up the CRISPR system will snip target DNA only where you want them to. the item can be also incredibly cheap. Unlike previous gene editing systems which could cost thousands of dollars, a relative novice can purchase a CRISPR toolkit for less than US$50.

Research labs around the planet are within the process of turning the hype surrounding the CRISPR technique into real results. Addgene, a nonprofit supplier of scientific reagents, has shipped tens of thousands of CRISPR toolkits to researchers in more than 80 countries, as well as the scientific literature can be currently packed with thousands of CRISPR-related publications.

When you give scientists access to powerful tools, they can produce some pretty amazing results.

The CRISPR revolution in medicine

The most promising (as well as obvious) applications of gene editing are in medicine. As we learn more about the molecular underpinnings of various diseases, stunning progress has been made in correcting genetic diseases within the laboratory just over the past few years.

Take, for example, muscular dystrophy – a complex as well as devastating family of diseases characterized by the breakdown of a molecular component of muscle called dystrophin. For some types of muscular dystrophy, the cause of the breakdown can be understood at the DNA level.

In 2014, researchers at the University of Texas showed which CRISPR could correct mutations associated with muscular dystrophy in isolated fertilized mouse eggs which, after being reimplanted, then grew into healthy mice. By February of This particular year, a team here at the University of Washington published results of a CRISPR-based gene replacement therapy which largely repaired the effects of Duchenne muscular dystrophy in adult mice. These mice showed significantly improved upon muscle strength – approaching normal levels – four months after receiving treatment.

Using CRISPR to correct disease-causing genetic mutations can be certainly not a panacea. For starters, many diseases have causes outside the letters of our DNA. as well as even for diseases which are genetically encoded, creating sense of the six billion DNA letters which comprise the human genome can be no modest task. although here CRISPR can be again advancing science; by adding or removing completely new mutations – or even turning whole genes on or off – scientists are beginning to probe the basic code of life like never before.

CRISPR can be already showing health applications beyond editing the DNA in our cells. A large team out of Harvard as well as MIT just debuted a CRISPR-based technology which enables precise detection of pathogens like Zika as well as dengue virus at extremely low cost – an estimated $0.61 per sample.

Using their system, the molecular components of CRISPR are dried up as well as smeared onto a strip of paper. Samples of bodily fluid (blood serum, urine or saliva) can be applied to these strips within the field as well as, because they linked CRISPR components to fluorescent particles, the amount of a specific virus within the sample can be quantified based on a visual readout. A sample which glows bright green could indicate a life-threatening dengue virus infection, for instance. The technology can also identify between bacterial species (useful for diagnosing infection) as well as could even determine mutations specific to an individual patient’s cancer (useful for personalized medicine).

Beyond just promise, CRISPR can be delivering within the lab today

Almost all of CRISPR’s advances in improving human health remain in an early, experimental phase. We may not have to wait long to see This particular technology make its way into actual, living people though; the CEO of the biotech company Editas has announced plans to file paperwork with the Food as well as Drug Administration for an investigational completely new drug (a necessary legal step before beginning clinical trials) later This particular year. The company intends to use CRISPR to correct mutations in a gene associated with the most common cause of inherited childhood blindness.

CRISPR will soon affect what we eat

Physicians as well as medical researchers are not the only ones interested in creating precise improvements to DNA. In 2013, agricultural biotechnologists demonstrated which genes in rice as well as different crops could be modified using CRISPR – for instance, to silence a gene associated with susceptibility to bacterial blight. Less than a year later, a different group showed which CRISPR also worked in pigs. In This particular case, researchers sought to modify a gene related to blood coagulation, as leftover blood can promote bacterial growth in meat.

You won’t find CRISPR-modified food in your local grocery store just yet. As with medical applications, agricultural gene editing breakthroughs achieved within the laboratory take time to mature into commercially viable products, which must then be determined to be safe. Here again, though, CRISPR can be changing things.

A common perception of what the item means to genetically modify a crop involves swapping genes through one organism to another – putting a fish gene into a tomato, for example. While This particular type of genetic modification – known as transfection – has actually been used, there are different ways to change DNA. CRISPR has the advantage of being much more programmable than previous gene editing technologies, meaning very specific improvements can be made in just a few DNA letters.

This particular precision led Yinong Yang – a plant biologist at Penn State – to write a letter to the USDA in 2015 seeking clarification on a current research project. He was within the process of modifying an edible white mushroom so the item might brown less on the shelf. This particular could be accomplished, he discovered, by turning down the volume of just one gene.

Yang was doing This particular work using CRISPR, as well as because his process did not introduce any foreign DNA into the mushrooms, he wanted to know if the product might be considered a “regulated article” by the Animal as well as Plant Health Inspection Service, a division of the U.S. Department of Agriculture tasked with regulating GMOs.

“APHIS does not consider CRISPR/Cas9-edited white button mushrooms as described in your October 30, 2015 letter to be regulated,” they replied.

Yang’s mushrooms were not the first genetically modified crop deemed exempt through current USDA regulation, although they were the first made using CRISPR. The heightened attention which CRISPR has brought to the gene editing field can be forcing policymakers within the U.S. as well as abroad to update some of their thinking around what the item means to genetically modify food.

completely new frontiers for CRISPR

One particularly controversial application of This particular powerful gene editing technology can be the possibility of driving certain species to extinction – such as the most lethal animal on Earth, the malaria-causing Anopheles gambiae mosquito. This particular can be, as far as scientists can tell, actually possible, as well as some serious players like the Bill as well as Melinda Gates Foundation are already investing within the project. (The BMGF funds The Conversation Africa.)

Most CRISPR applications are not nearly as ethically fraught. Here at the University of Washington, CRISPR can be helping researchers understand how embryonic stem cells mature, how DNA can be spatially reorganized inside living cells as well as why some frogs can regrow their spinal cords (an ability we humans do not share).

the item can be safe to say CRISPR can be more than just hype. Centuries ago we were writing on clay tablets – in This particular century we will write the stuff of life.


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Modifying fat content in soybean oil with the molecular scissors Cpf1

Journal reference:
Science

Provided by:
The Conversation

Beyond just promise, CRISPR can be delivering within the lab today

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